Figure 7
From: Knockdown of lncRNA MALAT1 attenuates renal interstitial fibrosis through miR-124-3p/ITGB1 axis
(a) Pathological changes were analyzed using HE staining in ON induced RIF clinic samples(bar = 50 um). (b, c) The extent of collagen deposition in RIF was assessed with Masson staining (bar = 50 um). ImageJ image analysis revealing that the percentage of the positive area of collagen deposition in clinical kidney specimen (n = 20). ****: p < 0.0001 (d) qRT-PCR showed that lncRNA MALAT1and ITGB1 were upregulated in ON induced RIF clinic samples, while miR-124-3p was downregulated. ***: p < 0.001. (e, f, g) WB was performed to determine the protein expression of FN, COL 1, VIM, α-SMA, ITGB1 in clinic renal tissues. ****: p < 0.0001 h, (i) IHC staining for FN, COL 1, α-SMA, ITGB1expression in clinic renal tissues(bar = 50 um). semi-quantitative data showing the relative expression of FN, COL 1, α-SMA and ITGB1 in each group (n = 20). **: p < 0.01; ***: p < 0.001.
