Figure 1

Experimental set up for ischemia induction and EIM measurement in the right hind limb. After the skin is shaved, electrodes are attached to the skin over the anterolateral muscle compartment (a). Size 3 neonatal blood pressure cuff is placed around the leg (b). Elastic rubber band is tightened around the upper thigh and the blood pressure cuff is inflated to 300 mmHg (c).