Figure 2
From: Early to sustained impacts of lethal radiation on circulating miRNAs in a minipig model
(a) Percentage share of the network categories across the temporal phases. The stacked bar plots represents the six dominating categories of networks. The group of networks labeled ‘others’ were linked to cell movement, cell cycle, neurological illness, developmental disorder etc. (b) Top five most significantly perturbed networks linked to Early time point. There were 37 networks that were consistently enriched across all four temporal phases, i.e. from Early to LR. Likewise, there were 41 and 4 networks that were consistently enriched in two other chronological combinations of temporal phases, i.e. from Early to Late and from Early to Mid, respectively. These networks were arranged in ascending order of their hypergeometric p values and five top ranked networks were selected from the two combinations of temporal phases, i.e. from Early to LR, and from Early to Late, respectively. All four networks from the third combination, i.e. from Early to Mid were selected. These networks emerged significantly enriched in at least two chronologically consecutive phases starting from the Early phase. The bar graphs were plotted against the top x-axis represents –log(p-value) and the solid black circles plotted against the bottom x-axis represents the number of miRNAs enriching individual networks. (c) Network linked to gene silencing. A regulatory network linked to the noncoding RNA residing in the homeobox C (HOXC) locus, termed HOTAIR emerged significantly altered within this framework of gene silencing network. In the network, the rectangular and oval nodes represent biofucntions and miRNAs, respectively. The four segments of the bar at the bottom of each miRNAs documented the regulations in four temporal phases arranged chronologically from left to right. The red, green and yellow colored segments represented up-, down and unchanged regulations in comparison to the pre-TBI baseline. The edges represent the relationship between two nodes; the arrow-headed edges represent activation and the straight-lined edges represent association between two nodes.
