Table 1 Biosafety of PAA-CeO nanoparticles in UN- and RA-SH-SY5Y cells.

From: Neuroprotective effects of polyacrylic acid (PAA) conjugated cerium oxide against hydrogen peroxide- and 6-OHDA-induced SH-SY5Y cell damage

Sample

UN-SH-SY5Y

RA-SH-SY5Y

LDH release (% Control)

PI-positive cells (%)

LDH release (% Control)

PI-positive cells (%)

Control + Vehicle

99.99 ± 0.00

13.10 ± 3.07

99.99 ± 0.00

4.96 ± 1.29

0.75 mM

99.53 ± 5.59

n.d.

97.56 ± 1.63

n.d.

1.5 mM

107.87 ± 5.63

n.d.

103.99 ± 5.39

n.d.

3 mM

107.59 ± 6.11

10.17 ± 1.04

104.52 ± 3.32

2.46 ± 0.80

  1. The UN- and RA-SH-SY5Y cells were treated with PAA-CeO (0.75–3 mM) or vehicle for 24 h. Cell cytotoxicity was measured in a culture medium with LDH release assay and in the cells by propidium iodide (PI) staining. The LDH data were normalized to the vehicle-treated cells, whereas PI data are shown as a percentage of PI-positive cells. The data were analyzed by one-way ANOVA followed by Duncan’s posthoc test and are presented as the mean ± SEM from 4-8 independent experiments. n.d.-not determined.
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