Table 2 Effects of PAA-CeO on the H2O2-induced caspase-3 activity in UN- and RA-SH-SY5Y cells.

From: Neuroprotective effects of polyacrylic acid (PAA) conjugated cerium oxide against hydrogen peroxide- and 6-OHDA-induced SH-SY5Y cell damage

Sample

UN-SH-SY5Y

9 h

RA-SH-SY5Y

9 h

RA-SH-SY5Y

18 h

Control + Vehicle

100.00 ± 0.00

100.00 ± 0.00

100 ± 0.00

H2O2 + Vehicle

344.46 ± 0.00***

185.48 ± 0.00***

699.60 ± 2.40***

H2O2 + PAA-CeO

342.52 ± 25.11***

182.07 ± 17.85***

734.42 ± 63.17***

H2O2 + Ac-DEVD-CHO

2.94 ± 0.87***, ###

1.92 ± 0.77***, ###

n.d.

PAA-CeO

77.56 ± 12.08

33.06 ± 4.77***

n.d.

  1. The UN- and RA-SH-SY5Y cells were pretreated for 30 min with PAA-CeO (3 mM) followed by 9 h of treatment with H2O2 (0.375 and 0.5 mM for UN- and RA-SH-SY5Y, respectively). For RA-SH-SY5Y cells caspase-3 activity was also measured 18 h after H2O2 (0.5 mM) treatment. Ac-DEVD-CHO (20 μM) was used as a positive control for the assay. Data were normalized to vehicle-treated cells (control) and are presented as the mean ± SEM from 2–3 independent experiments with 2 replicates each. ***P < 0.001 versus vehicle-treated cells; ###P < 0.001 versus H2O2-treated cells. n.d.-not determined.
Back to article page