Figure 2

PAD4 deficiency does not rescue DC-specific A20 deficient mice from developing SLE pathology. (A) Breeding scheme to generate A20^DC-KO and A20^DC-KOPadi4^−/− mice. (B, C) Body weight (B) and spleen weight (C) of 25–30 week old wild-type, A20^DC-KO, Padi4^−/− and A20^DC-KOPadi4^−/− mice. Each dot represents one mouse. Data are expressed as mean ± s.e.m. (D) Serum levels of TNF, IL-6, IL-22 and BAFF in indicated genotypes. (E) Quantitative PCR results for interferon-stimulated genes on whole spleen of mice with indicated genotypes. (F) Serum auto-antibody concentrations on ELISA in mice with indicated genotypes. (G) Representative immunofluorescent image of glomerular IgA deposition per genotype. White dotted circles indicate the glomeruli. (H) Quantification of the glomerular mean fluorescent signal for IgA. (I) Representative hematoxylin and eosin-stained kidneys of mice with the indicated genotypes, showing extensive perivascular infiltrates in both A20^DC-KO and A20^DC-KOPadi4^−/− mice. Each dot represents one mouse. Data are expressed as mean. Each dot represents a biologically independent mouse. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Scalebar, 50 µm. CNRQ = Calibrated Normalized Relative Quantity. Results are representative of two independent experiments.