Figure 2

Effects of SCFAs on immune cell subsets in the liver during FAdV-4 infection. The frequencies of macrophages/monocytes and T cell subsets in liver were determined using flow cytometry at 2 dpi. (A) MHCII+ macrophages/monocytes were examined. Representative plots are shown. (B–D) Frequencies of CD44-expressing T cells (CD3+, CD4+, and CD8+) were calculated and representative plots are shown. Cells were obtained from 3 to 5 chickens per group. At least three independent experiments were performed, and pooled data are shown. Significant differences were identified using one way ANOVA. (*p < 0.05).