Table 4 Description of genetic profile of keratinocyte cells after cellular target detection.

From: Targeting cell-derived markers to improve the detection of invisible biological traces for the purpose of genetic-based criminal identification

 

Keratincoyte cells

Usability of profile

Degradation index

Number of missing loci

Amelogenin

Q/S

Control

2/2

1.2

1

0/22

0/22

X–Y

Q/S

Dapi

Con A

2/2

0.8

1

0/22

0/22

X–Y

Q/S

PNA

2/2

1.2

1.5

0/22

0/22

X–Y

Q/S

SNA

2/2

1.2

1.2

0/22

0/22

X–Y

Q/S

UEA

2/2

1

1

0/22

0/22

X–Y

Q/S

Lam

2/2

0.8

1.3

0/22

0/22

X–Y

Q/S

K10

2/2

1.1

0.8

0/22

0/22

X–Y

Q/S

Hoechst

Con A

2/2

1.2

1

0/22

0/22

X–Y

Q/S

PNA

2/2

1.1

1.3

0/22

0/22

X–Y

Q/S

SNA

2/2

0.9

1

0/22

0/22

X–Y

Q/S

UEA

2/2

1.2

2

0/22

0/22

X–Y

Q/S

Lam

2/2

1.1

0.9

0/22

0/22

X–Y

Q/S

K10

2/2

1

0.9

0/22

0/22

X–Y

Q/S

Anti-H2B

Con A

2/2

1

1.1

0/22

0/22

X–Y

Q/S

PNA

2/2

0.9

1

0/22

0/22

X–Y

Q/S

SNA

2/2

1.1

0.9

0/22

0/22

X–Y

Q/S

UEA

2/2

0.8

1.3

0/22

0/22

X–Y

Q/S

Lam

2/2

1.2

1

0/22

0/22

X–Y

Q/S

K10

2/2

1.5

1

0/22

0/22

X–Y

Q/S

  1. The height of the peaks was measured in rfu (relative fluorescence unit): it was proportional to the quantity of PCR products detected. A profile was usable if the number of complete Short Tandem Repeat (STR) markers was greater than 5, according to the requirements of the French database Fichier National Automatisé Empreintes Génétiques. A profile was complete if all STR markers (22 STR) were amplified and present. Degradation index indicated the quality of the DNA (value close to 0: complete degradation; value close to 1: no degradation). Human gender determination was performed by analyzing the amelogenin gene which is common to both X and Y chromosomes. If the two quality sensors (Q and S) were visible on the electrophoregram, it meant that the PCR had been successful and there was no inhibition. 2/2 indicates the number of usable profiles per replicate. Results are representative of at least two independent experiments.
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