Table 5 Description of genetic profile of fingermarks after cellular target detection.

From: Targeting cell-derived markers to improve the detection of invisible biological traces for the purpose of genetic-based criminal identification

 

Fingermark samples

Usability of profile

Degradation index

Number of missing loci

Amelogenin

Q/S

Control

3/3

1.1

0.8

0.7

5/22

4/22

4/22

X-X

Q/S

Dapi

Con A

2/3

0.8

0.9

1.8

5/22

5/22

19/22

X-X

Q/S

PNA

3/3

1.2

0.8

0.8

17/22

17/22

10/22

X-X

Q/S

SNA

3/3

0.8

0.8

0.9

13/22

17/22

16/22

X-X

Q/S

UEA

3/3

1.5

1

0.8

14/22

16/22

10/22

X-X

Q/S

Lam

3/3

0.7

0.7

0.8

14/22

12/22

16/22

X-X

Q/S

K10

2/3

0.9

0.8

0.7

15/22

14/22

20/22

X-X

Q/S

Hoechst

Con A

1/3

0.8

0.7

0.9

20/22

1/22

18/22

X-X

Q/S

PNA

3/3

0.7

0.9

0.7

16/22

2/22

2/22

X-X

Q/S

SNA

2/3

0.7

0.7

0.7

21/22

9/22

3/22

X-X

Q/S

UEA

2/3

0.8

0.7

0.7

14/22

5/22

19/22

X-X

Q/S

Lam

3/3

0.7

0.7

0.7

17/22

11/22

10/22

X-X

Q/S

K10

1/3

0.9

0.7

0.7

18/22

9/22

22/22

X-X

Q/S

Anti-H2B

Con A

3/3

0.8

0.6

1

16/22

15/22

12/22

X-X

Q/S

PNA

2/3

0.7

0.8

1

1/22

18/22

11/22

X-X

Q/S

SNA

3/3

0.7

0.8

1.2

16/22

9/22

16/22

X-X

Q/S

UEA

1/3

0.7

0.9

0.7

18/22

19/22

3/22

X-X

Q/S

Lam

3/3

0.7

0.7

1

2/22

8/2

17/22

X-X

Q/S

K10

0/3

0.7

0.7

0.8

20/22

18/22

18/22

X-X

Q/S

  1. The height of the peaks was measured in rfu (relative fluorescence unit): it was proportional to the quantity of PCR products detected. A profile was usable if the number of complete Short Tandem Repeat (STR) markers was greater than 5, according to the requirements of the French database Fichier National Automatisé Empreintes Génétiques. A profile was complete if all STR markers (22 STR) were amplified and present. Degradation index indicated the quality of the DNA (value close to 0: complete degradation; value close to 1: no degradation). Human gender determination was performed by analyzing the amelogenin gene which is common to both X and Y chromosomes. If the two quality sensors (Q and S) were visible on the electrophoregram, it means that the PCR was successful and there was no inhibition. 3/3 indicates the number of usable profiles per replicate. Results are representative of at least three independent experiments.
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