Figure 2 | Scientific Reports

Figure 2

From: HOPS, CORVET and newly-identified Hybrid tethering complexes contribute differentially towards multiple modes of endocytosis

Figure 2The alternative text for this image may have been generated using AI.

“Hybrid” complexes composed of the subunits of HOPS/CORVET. (a,b) Schematic representation of HOPS and CORVET (a), and non-canonical tethering complexes named Hybrid-A and Hybrid-B (b) interacting with Rab5- or Rab7-positive vesicles. (c) Experimental strategies to reconstitute and immunoprecipitate the tethering complexes with over-expressed subunits with different tags. (d,e) The HOPS/CORVET subunits which can form a complex with Myc-VPS41 (d) or Myc-VPS8 (e) were determined by immunoprecipitation (IP) analyses. A combination of the introduced plasmids for each lane is shown on the top of the blots. The left half of each panel shows input control without IP. The expected bands of each blot were indicated by arrowheads on the right side. The name of the complex corresponding to each band combination detected after IP is shown at the bottom. An asterisk (*) represents a non-specific band.

Back to article page