Figure 2
m6A modification level of AC026356.1 in HCC and its correlation with prognosis. (A) m6A modification levels of AC026356.1 in immortalized liver cell line THLE-2 and HCC cell lines HuH-7, SNU-398, HCCLM3, and Hep3B were detected using MeRIP-qPCR assays. Data are shown as mean ± SD of three independent experiments. #P < 0.01 by Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way ANOVA followed by Dunnett's multiple comparisons test. (B) m6A modification levels of AC026356.1 in 65 pairs of HCC tissues and matched noncancerous liver tissues were detected using MeRIP-qPCR assays. Data are shown as median with interquartile range. P values were calculated using Wilcoxon matched-pairs signed rank test. (C) Kaplan–Meier analysis of the correlation between m6A modification level of AC026356.1 and overall survival in HCC (n = 65). Median m6A modification level of AC026356.1 was used as cut-off. HR and P values were calculated using log-rank test.
