Figure 3
m6A modification increases AC026356.1 transcript stability. (A) The correlation between AC026356.1 expression level and m6A modification level of AC026356.1 in HCC tissues (n = 65). P and r values were calculated using Spearman correlation analysis. (B–E) The correlation between AC026356.1 expression level and WTAP (B), METTL3 (C), METTL14 (D), or METTL16 (E) expression levels in HCC tissues (n = 371) based on TCGA LIHC data. P and r values were calculated using Spearman correlation analysis. (F) m6A modification levels of AC026356.1 in SNU-398 and HCCLM3 cells with WTAP or METTL16 overexpression were detected using MeRIP-qPCR assays. (G,H) The stability of AC026356.1 transcript in SNU-398 (G) and HCCLM3 (H) cells with WTAP or METTL16 overexpression over time was detected after blocking new RNA synthesis with α-amanitin (50 µM) and normalized to 18S rRNA (a product of RNA polymerase I that is unchanged by α-amanitin). (I) m6A modification levels of AC026356.1 in SNU-398 and HCCLM3 cells with WTAP or METTL16 depletion were detected using MeRIP-qPCR assays. (J,K) The stability of AC026356.1 transcript in SNU-398 (J) and HCCLM3 (K) cells with WTAP or METTL16 depletion over time was detected after blocking new RNA synthesis with α-amanitin (50 µM) and normalized to 18S rRNA. For (F–K), data are shown as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 by one-way ANOVA followed by Dunnett's multiple comparisons test.
