Figure 5
Loss of m6A modification abolished the oncogenic roles of AC026356.1 in HCC. (A) AC026356.1 expression in HCCLM3 cells with AC026356.1 overexpression and concurrent WTAP and METLL16 depletion was measured by qPCR. (B) m6A modification levels of AC026356.1 in HCCLM3 cells with WTAP and METTL16 depletion were detected using MeRIP-qPCR assays. (C) Cell proliferation of HCCLM3 cells with AC026356.1 overexpression and concurrent WTAP and METLL16 depletion was detected using CCK-8 assays. (D) Cell proliferation of HCCLM3 cells with AC026356.1 overexpression and concurrent WTAP and METLL16 depletion was detected using EdU incorporation assay. Scale bars 100 µm. (E) Cell migration of HCCLM3 cells with AC026356.1 overexpression and concurrent WTAP and METLL16 depletion was detected using transwell migration assay. Scale bars 100 µm. Data are shown as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant, by Student’s t-test.
