Figure 6
AC026356.1 upregulates IL11 in an m6A-depedent manner. (A–D) GSEA of KEGG JAK-STAT signaling pathway and WikiPathways STAT3 signaling pathway gene signatures (A), STAT3 transcription factor bound gene signature (B), STAT3-upregulated gene signatures (C), and WikiPathways IL11 signaling pathway gene signature (D) in AC026356.1 high expression group versus AC026356.1 low expression group. NES, normalized enrichment score. (E) The correlation between AC026356.1 and IL11 expression levels in HCC tissues (n = 371) based on TCGA LIHC data. P and r values were calculated using Spearman correlation analysis. (F) IL11 expression in HCCLM3 cells with AC026356.1 overexpression was measured by qPCR. (G) IL11 expression in HCCLM3 cells with AC026356.1 overexpression and concurrent WTAP and METLL16 depletion was measured by qPCR. (H) IL11 expression in HCCLM3 cells with AC026356.1 silencing was measured by qPCR. (I) IL-11 concentration in the culture medium of HCCLM3 cells with AC026356.1 overexpression was measured by ELISA. (J) IL-11 concentration in the culture medium of HCCLM3 cells with AC026356.1 overexpression and concurrent WTAP and METLL16 depletion was measured by ELISA. (K) IL-11 concentration in the culture medium of HCCLM3 cells with AC026356.1 silencing was measured by ELISA. For (F–K), data are shown as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant by Student’s t-test (F,G,I,J) or one-way ANOVA followed by Dunnett's multiple comparisons test (H,K).
