Figure 1 | Scientific Reports

Figure 1

From: IL-8 is a novel prometastatic chemokine in intrahepatic cholangiocarcinoma that induces CXCR2-PI3K/AKT signaling upon CD97 activation

Figure 1

CD97 molecules are the targets of IL-8 and associate with ICC. Protocol for preparing cell samples for RNA sequencing. Volcano plot of RNA-seq results for the QBC-939 cell line in either the absence or presence of IL-8. Upregulated and downregulated genes are indicated as red and green dots, respectively (A). Enrichment analysis results of all DEGs by from GO (B) and KEGG pathway (C) enrichment analysis. Immunohistochemical staining showed that the expression and location of IL-8 and CD97 were consistent in ICC and hepatolithiasis tissues (D). Kaplan‒Meier survival curve analysis according to IL-8 and CD97 expression in ICC (E, log-rank test). CD97 expression in QBC-939 cells and RBE cells after treatment with different doses of IL-8 (0, 25, 50, 100, 200 ng/mL) and different exposure times (24 h, 48 h) (F, G). *P < 0.05, **P < 0.01, ***P < 0.001, #P > 0.05.

Back to article page