Figure 6
From: Insights into next generation sequencing guided antibody selection strategies
Correlation of NGS to Affinity. Scatterplot of relative abundance versus affinity for (a) top representative in given cluster, (b) all unique HCDR3, (c) all unique concatenated HCDR3 + LCDR3, and (d) concatenated CDRs. Scatterplot of fold enrichment (10 nM to 1 nM) versus affinity for (e) top representative in given cluster, (f) all unique HCDR3, (g) all unique concatenated HCDR3 + LCDR3, and (h) concatenated CDRs. The number of additional clones in each cluster with lower relative abundance for the full-length sequence with improved, similar or worse affinities using (i) twofold and (j) fivefold threshold. The number of additional clones in each cluster with lower fold enrichment for the full-length sequence with improved, similar or worse affinities using (k) twofold and (l) fivefold threshold. (m) Paired sequence profile of the HCDR3 clusters (x-axis) versus the LCDR3 clusters (y-axis). All selected antibodies are color-coded according to their corresponding bin group, except gray which are the non-selected NGS clones. The size of dot is reflective of number of additional full-length sequences within a given cluster. Two additional boxplots show the affinity range across each respective cluster with top boxplot showing same HCDR3 clusters paired with different LCDR3 clusters. Right boxplot shows same LCDR3 cluster paired to many different HCDR3 clusters. Boxplot are color coded to show the number of unique HCDR3 and LCDR3 in the cluster population.
