Figure 2 | Scientific Reports

Figure 2

From: Gene therapy for alopecia in type II rickets model rats using vitamin D receptor-expressing adenovirus vector

Figure 2

Effects of infection of VDR-AdV to keratinocyte primary cells prepared from Vdr-KO rats on expression of VDR protein, and induction of Cyp24a1 mRNA by adding 1,25D3. (A) Western blot analysis of VDR in the VDR-AdV noninfected (lane 1) and infected (lane 2) keratinocyte primary cell of Vdr-KO rats. (See Supplemental Fig. 4). (B) Immunostaining of VDR in the VDR-AdV noninfected (VDR-AdV ( −)) and infected (VDR-AdV ( +)) cells, and nuclear translocation of VDR by adding 10 nM 1,25D3 in the cell culture (VDR-AdV( +) 1,25(OH)2D3( +)). Scale bar; 200 µm. (C) Outline of the mechanism of VDR-mediated induction of Cyp24a1. (D) Transcriptional induction of Cyp24a1 by adding 10 nM 1,25D3 in VDR-AdV infected Vdr-KO rat primary cells. (n = 3, p = 0.00441).

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