Table 5 The values from bioimage analysis after 4 h exposition of HeLa cell lines to 100 µM lilial with or without the S9 mix compared to unaffected, negative control (NC).

From: Toxicological investigation of lilial

Conditions

Sample

MoA

Fold

RA (%)

Mean

IQR

Mean

IQR

4 h

NC

0.05

1.00

 

100.0

 

4 h

Lilial

0.11

2.15

1.41

86.1

23.5

4 h

PC

0.13*

2.63

0.49

95.8

3.9

4h_S9

NC

0.10

1.00

 

100.0

 

4h_S9

Lilial

0.20

2.03

0.26

94.2

4.3

4h_S9

PC

0.29**

2.94

0.06

97.8

3.0

24 h

NC

0.02

1.00

 

100.0

 

24 h

Lilial

0.03

1.27

1.34

94.8

23.5

24 h

PC

0.55*

24.03

4.33

101.5

3.9

  1. 30 μM valinomycin was used as a positive control (PC).
  2. MoA mean fluorescence values over area of the nuclei, IQR interquartile range, RA relative area of nuclei (analogue of relative cell count). Fold values above normative limit of 1.5 are in bold. Statistical significance was verified by Kruskal–Wallis test followed by post-hoc Dunn’s test with Bonferroni correction—MoA comparison with NC (*p ˂ 0.05, **p ˂ 0.01, ***p˂ 0.001).
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