Figure 3 | Scientific Reports

Figure 3

From: Differential effects of interleukin-17A and 17F on cell interactions between immune cells and stromal cells from synovium or skin

Figure 3

Effects of exogenous cytokines and antibodies on IL-17 receptor expression in co-cultures between PBMC and stromal cells from different origins. PHA-activated PBMC were treated or not with exogenous cytokines (IL-23, IL-17A, IL-17A/F and IL-17F) or antibodies (anti-IL-17A, anti-IL-17A/F and anti-IL-17F) and co-cultured with synoviocytes (A–D) or with skin fibroblasts (E–H) at a 5:1 ratio for 24 h. RNA was recovered and IL-17RA, IL-17RC, IL-23R, IL-12Rβ1 and IL-12Rβ2 expression was assessed by RT-QPCR. The results show the gene expression normalized by GAPDH expression. *p < 0.05; **p < 0.01. Receptor expression was expressed as a percentage of change compared to the control condition, which is the non-treated condition (NT) and used as 100% control. Results are presented as mean ± SD, n = 5 to 6 experiments.

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