Figure 4

Effects of exogenous cytokines and antibodies on IL-17A and IL-17F production in co-cultures between PBMC or PBL and stromal cells from different origins. PHA-activated PBMC or PBL were treated or not with exogenous cytokines (IL-23, IL-17A, IL-17A/F and IL-17F) or antibodies (anti-IL-17A, anti-IL-17A/F and anti-IL-17F) and co-cultured with synoviocytes (A–D) or with skin fibroblasts (E–H) at a 5:1 ratio for 48 h. Production of IL-17A and IL-17F was measured by ELISA. “*” Compares the effect of the treatments or antibodies vs. the control condition. *p < 0.05; **p < 0.01; ***p < 0.005. “^#” Compares the effect of monocyte exclusion on cytokine production (PBMC vs. PBL) within the same treatment condition. ^#p < 0.05; ^##p < 0.025. “•” Compares the effect of interaction with stromal cell from different origin on cytokine production (synoviocyte vs. skin fibroblast). ^nsp > 0.05; ^•p < 0.05. Results are represented as mean ± SD in pg/mL, n = 4 to 7 experiments.