Figure 2 | Scientific Reports

Figure 2

From: Chloroplast transformation for bioencapsulation and oral delivery using the immunoglobulin G fragment crystallizable (Fc) domain

Figure 2

Quantification of GFP and GFP-Fc1 accumulation in transplastomic TVV tobacco leaves. (a) Quantification of GFP in the pMRR13 line by 1D-Multilane Densitometry (Alphaimager 2200, ProteinSimple, San Jose, CA, USA). Leaf extracts were separated in SDS-PAGE (12.5%) and stained with Coomassie brilliant blue. (b) Quantification of GFP-Fc1 in the TVV2 line on immunoblots, using GFP Ab and a dilution of pMRR13 extract as reference. GFP-Fc1 abundance was measured against GFP in the MRR13 reference line (four biological replicates; mean ± 1 SD) with the ImageJ software (version 2.0.0-rc-38/1.50b) (c) Quantification of proIGF-I-GGG-Fc1 in the TVV3 line and proIGF-I-2xFurin-Fc1 in TVV3 line on immunoblots, using Fc Ab and a dilution of TVV2 extract as reference. Protein concentrations were calculated with the ImageJ software (version 2.0.0-rc-38/1.50b; four biological replicates; mean ± 1 SD). LSU, rubisco large subunit; SSU, rubisco small subunit.

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