Table 2 Pan-rodent Ppia and viral target multiplex assay primer sequences.
From: Optimal reference genes for RNA tissue analysis in small animal models of hemorrhagic fever viruses
Multiplex assay primer sequences | ||
|---|---|---|
Oligo name | Primer sequence (5′-3′) | Conc. (µM) |
Pan_Ppia_Fwd | CCCACCGTGTTCTTCGAC | 400 |
Pan_Ppia_Rev | TCCTTTCTCTCCAGTGCTCAG | 400 |
Pan_Ppia_Prb | GAGCCCTTGGGCCGCGTCTC | 200 |
CCHFV_Fwd | CAGGACATGGACATAGTGGC | 400 |
CCHFV_Rev | ATTGCCCTTGACGTTGTAGG | 400 |
CCHFV_Prb | CCCTTGTTGGCAAGCAATCCC | 200 |
NiV_Fwd | CTGGTCTCTGCAGTTATCACCATCGA | 400 |
NiV_Rev | ACGTACTTAGCCCATCTTCTAGTTTCA | 400 |
NiV_Prb | CAGCTCCCGACACTGCCGAGGAT | 200 |
LASV_Fwd | GGAAGCCACAGAAAGCTGAC | 400 |
LASV_Rev | GGAGTGCATCAATGACAGCA | 400 |
LASV_Prb | AAATCCCTGCAGTCGGCAGG | 200 |
