Figure 5
Overexpression of STING mimics hDT806’s stimulation of the STING-IFN-I axis in HNSCC cells. (A) Total protein extracts were prepared from the JHU-029 cells with (designated as JHU029-STING) or without (designated as JHU029-control) STING overexpression. Western blot analysis was performed for STING (a), p-TBK1 (b), TBK1 (c), p-p38 (d), p38 (e), IFNB (f), CXCL10 (g), MX1 (h), and β-actin (i). (B) Band intensities of STING (a), IFNB (d), CXCL10 (e), and MX1 (f) relative to the corresponding β-actin, the ratios of p-TBK1/TBK1 (b) and p-p38/p38 (c) were quantified for comparisons between the JHU029-STING and JHU029-control groups. Data of three or four independent experiments are presented as mean ± SEM, with p values indicated.
