Figure 1 | Scientific Reports

Figure 1

From: Proteomic profile of naturally released extracellular vesicles secreted from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses

Figure 1

Characterization of native EVs from Leptospira interrogans serovar Pomona exposed to three culture conditions; in vitro EMJH medium at 30 °C, temperature shifted to 37 °C, and physiologic osmolarity at 30 °C with added 120 mM NaCl. Six biological replicates of each culture condition were performed. After sucrose gradient centrifugation, the enriched EVs in the selected sucrose fractions were pooled before characterization using transmission electron microscopy with negative staining (a), and dynamic light scattering (b). A, the EV samples were stained with UranyLess EM Stain and observed under JEM 1400 transmission electron microscope. B, the particle size distribution was measured using NanoSight NS300. The results represent mean of triplicate measurement with standard deviation of each culture condition. These figures are representative of the results obtained from the six biological replicates.

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