Figure 3 | Scientific Reports

Figure 3

From: Proteomic profile of naturally released extracellular vesicles secreted from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses

Figure 3

Absolute quantification of proteins in native EVs from Leptospira interrogans serovar Pomona exposed to three culture conditions; in vitro EMJH medium at 30 °C, temperature shifted to 37 °C, and physiologic osmolarity at 30 °C with added 120 mM NaCl. Six biological replicates of each culture condition were performed. The proteins repeatedly identified by at least 3 out of 6 replicates were further analyzed. The proteins identified in the EVs were quantitated using intensity-based absolute quantification (iBAQ). The sum of iBAQ values for each subcellular compartment (a) and COG functional category (b) were compared. The 30 most abundant proteins in the EVs obtained from each culture condition are shown (c). A and C, subcellular localization includes extracellular (EC), outer membrane (OM), periplasm (PP), inner membrane (IM), cytoplasm (CP), and unknown (Unk).

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