Figure 4

Relative quantification of proteins in native EVs from Leptospira interrogans serovar Pomona exposed to stress conditions; temperature shifted to 37 °C and physiologic osmolarity at 30 °C with added 120 mM NaCl compared with culture in EMJH medium at 30 °C. Six biological replicates of each culture condition were performed. The proteins repeatedly identified by at least 3 out of 6 replicates were further analyzed. The volcano plots show the distribution of the identified proteins in EVs according to -log10 (p-value) on the y-axis and log2 ratio on the x-axis. The log2 ratio is the comparison between temperature shift (37 °C/30 °C) (a) or osmolarity shift (30 °C + 120 mM NaCl/30 °C) (b) and culture at 30 °C. The log2 ratio of each comparison was compared with a value of 0, which represents no change (log2 of 1). The significant difference was calculated using the Student's t-test. Each dot represents a protein. Letters next to the dots are the predicted COG functional category and the protein names are shown in brackets. A and B, the predicted subcellular localization includes extracellular (EC), outer membrane (OM), periplasm (PP), inner membrane (IM), cytoplasm (CP), unknown (Unk), and not significant (NS).