Figure 4

Metabolic adaptation features between two distinct IR patterns. (A) Differences in the proportions of upregulated, neutral, and downregulated TCA cycle metabolism, amino acid metabolism, and nucleotide metabolism between the two IR clusters in the training cohort (chi-square test). Orange represents upregulated metabolism, gray represents unchanged metabolism, and yellow represents downregulated metabolism. (B) Enrichment of metabolic pathways in distinct IR patterns was obtained through GSVA enrichment analysis. The bar chart displays differences in enrichment scores of these processes between the two IR patterns. (C) Expression levels of ARSs between the two IR clusters in the training cohort (Mann–Whitney U test). (D) Heatmap of genes linking effector T cell activation with metabolic pathways between the two IR patterns in the training cohort. Red represents high expression levels, and purple represents low expression levels. The IR cluster was used for patient annotations. Each column represents one patient, and each row represents one gene linking effector T cell activation with metabolic pathways. Asterisks represent the statistical P value (*P < 0.05; **P < 0.01; ***P < 0.001; ns no significance).