Figure 3
From: Early diagnosis of aortic calcification through dental X-ray examination for dental pulp stones
High concentrations of phosphate medium promoted calcification of DPCs. (A–D) Representative images of the cells at a higher magnification. Scale bar: 300 µm. Black box indicates a representative image of the well at a lower magnification. (E) Percentage of calcification area in SMCs and DPCs cultured with calcification medium supplemented with 6 mM Pi at day 5. Percentages were calculated based on the positive area of calcification per field, and results are shown as means ± standard errors of the mean (SEMs; 16–19 images in each culture condition). * p < 0.05 in comparisons between samples.
