Figure 4
From: Early diagnosis of aortic calcification through dental X-ray examination for dental pulp stones
High concentrations of phosphate medium resulted in upregulation of osteoblast differentiation markers in DPCs compared with SMCs. Real-time RT-PCR analysis results for Runx2 (A), BSP (B), and OCN (C) expression in SMCs and DPCs cultured in calcification medium supplemented with 3.0 and 6.0 mM Pi at day 1, 3, and 5. Biological triplicate experiments were analyzed. Fold changes in SMCs and DPCs relative to the control are shown. All values are means ± SEMs. * and ** indicate p < 0.05 and p < 0.001 in comparisons between samples, respectively. cont: cells cultured with DMEM containing 10% FBS (containing 0.9 mM Pi); 3 mM Pi: cells cultured in calcification medium supplemented with 3.0 mM Pi; 6 mM Pi: cells cultured in calcification medium supplemented with 6.0 mM Pi.
