Figure 2

Mitochondrial morphology and mass: electron microscopy and mitochondrial content markers. Electron microscopy (EM) imaging to identify changes in mitochondrial morphology (N = six images of N = 3/group). Shown are (A) typical examples of EM images focused on the mitochondria. (B) Mitochondrial number per field, (C) average area per mitochondria in squared micrometres (µm²), and (D) total mitochondrial area per field of 152 µm². Shown are (E) mitochondrial to nuclear DNA ratio (mtDNA/nDNA) (N = 7/group), (F) citrate synthase (CS) activity from homogenate samples in nanomole per minute per mg protein (nmol/min/mg) (N = 7/group) and (G) CS activity from isolated mitochondrial samples in nmol/min/mg (N = 7/group). Raw EM data with zoomable files at high resolution are accessible at www.nanotomy.org. ND1, NADH dehydrogenase 1; HK2, hexokinase 2; WT, wild type mice; AKIP1-TG, AKIP1 transgenic mice. Graphs represent mean ± standard error of the mean (SEM). Statistical analysis for comparing two groups was performed with Student’s t-test or Mann–Whitney U test. < 0.05 was considered statistically significant; p* < 0.05. Scale bars indicate 2 µm.