Figure 4 | Scientific Reports

Figure 4

From: Artificial surface labelling of Escherichia coli with StrepTagII antigen to study how monoclonal antibodies drive complement-mediated killing

Figure 4

IgM more efficiently triggers complement-mediated killing of MG1655-OmpX-StrepTagII. (a) MAC deposition, (b) % Sytox positive bacteria, and (c) Sytox intensity of MG1655-OmpX-StrepTagII bacteria that were incubated with a concentration range of anti-StrepTagII IgM or IgG1 at a fixed concentration of complement components of 1.25% serum equivalent as described in Fig. 1a. Samples were measured by flow cytometry and data represent mean ± SD of three independent experiments.

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