Figure 5 | Scientific Reports

Figure 5

From: Artificial surface labelling of Escherichia coli with StrepTagII antigen to study how monoclonal antibodies drive complement-mediated killing

Figure 5

Pre-formed hexameric IgG1 can achieve killing of MG1655-OmpX-StrepTagII as efficient as IgM. (a) Binding of a concentration range of anti-StrepTagII IgG1, IgG1 E430G, IgG1 RGY, IgM, and IgM C575A to either MG1655-OmpX-StrepTagII bacteria (solid lines) or for MG1655 Wt bacteria (dotted lines). (b) Complement-mediated killing (% Sytox positive) of MG1655-OmpX-StrepTagII after exposure to a concentration range of anti-StrepTagII IgG1, IgG1 E430G, IgG1 RGY, IgM, and IgM C575A at a fixed concentration of 1.25% serum equivalent complement components. Samples were measured by flow cytometry and data represent mean ± SD of three independent experiments.

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