Table 1 The half maximum effective concentration (EC50) of IgM is lower than that of IgG1.

From: Artificial surface labelling of Escherichia coli with StrepTagII antigen to study how monoclonal antibodies drive complement-mediated killing

 

EC50 IgG1 (SD)

EC50 IgM (SD)

EC50 IgG1 / EC50 IgM

Sytox influx (%) in µg/mL

3.14E−2 (1.61E−3)

2.43E−3 (9.10E−5)

12.9

Sytox influx (FI) in µg/mL

3.93E−2 (4.60E−3)

4.21E−3 (9.88E−4)

9.3

Average (µg/ml)

  

11.1

Sytox influx (%) in M

2.09E−10 (1.07E−11)

2.43E−12 (9.10E−14)

86.0

Sytox influx (FI) in M

2.62E−10 (3.07E−11)

4.21E−12 (9.89E−13)

62.3

Average (M)

  

74.2

  1. The EC50 values extracted from the four-parameter logistic curves fitted to the percentage of Sytox positive (%) bacteria and the GeoMFI-H Sytox data (FI). The upper rows are values for concentrations in µg/mL and lower rows are concentrations transformed to molar (M). For each extracted EC50 value, the standard deviation (SD) is provided between brackets. The right column shows the EC50 value for IgG1 divided over the EC50 value for IgM to obtain a ratio for the difference in EC50. The average of the values extracted from the % Sytox positive and GeoMFI-H Sytox were calculated and depicted in bold.
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