Table 2 The half maximum effective concentration (EC50) of engineered IgG molecules compared to IgG1.

From: Artificial surface labelling of Escherichia coli with StrepTagII antigen to study how monoclonal antibodies drive complement-mediated killing

 

EC50 IgG1 (SD)

EC50 E430G (SD)

EC50 RGY (SD)

EC50 ratio

Sytox influx (%) in M

2.09E−10 (1.41E−11)

1.13E−10 (1.02E−11)

 

1.9

Sytox influx (FI) in M

4.53E−10 (1.15E−10)

1.71E−10 (2.60E−11)

 

2.6

Average (IgG1/E430G)

   

2.3

Sytox influx (%) in M

2.09E−10 (1.41E−11)

 

4.34E−12 (4.57E−13)

48.1

Sytox influx (FI) in M

4.53E−10 (1.15E−10)

 

7.16E−12 (1.49E−12)

63.3

Average (IgG1/RGY)

   

55.7

  1. The EC50 values extracted from the four-parameter logistic curves fitted to the percentage of Sytox positive (%) bacteria and the GeoMFI-H Sytox data (FI). For each extracted EC50 value, the standard deviation (SD) is provided between brackets. The right column shows the EC50 value for IgG1 divided over the EC50 value for either IgG1 E430G or IgG1 RGY to obtain a ratio for the difference in EC50. The average of the values extracted from the % Sytox positive and GeoMFI-H Sytox were calculated and depicted in bold.
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