Table 5 Agreement between MCSeEd and qRT-PCR results.

From: Genome-wide epigenetic modifications in sports horses during training as an adaptation phenomenon

Gene

Comparison

MCSeEd outcome

RT-qPCR outcome

Agreement

AP5Z1

T30 vs. T0

N.S.

Down-regulation

 

T90 vs. T0

De-methylation

Up-regulation

Yes

T90 vs. T30

De-methylation

Up-regulation

Yes

ARFGAP2

T30 vs. T0

N.S.

Down-regulation

 

T90 vs. T0

De-methylation

N.S.

 

T90 vs. T30

De-methylation

Up-regulation

Yes

EVI5

T30 vs. T0

N.S.

N.S.

 

T90 vs. T0

De-methylation

N.S.

 

T90 vs. T30

De-methylation

N.S.

 

GRID1

T30 vs. T0

Methylation

N.S.

 

T90 vs. T0

De-methylation

N.S.

 

T90 vs. T30

N.S.

N.S.

 

LHX1

T30 vs. T0

Methylation

N.S.

 

T90 vs. T0

N.S.

N.S.

 

T90 vs. T30

N.S.

N.S.

 

RADIL

T30 vs. T0

N.S.

Down-regulation

 

T90 vs. T0

De-methylation

N.S.

 

T90 vs. T30

De-methylation

Up-regulation

Yes

ATP2A3

T30 vs. T0

Methylation

Down-regulation

Yes

T90 vs. T0

Methylation

N.S.

 

T90 vs. T30

N.S.

Up-regulation

 

RGS19

T30 vs. T0

Methylation

Down-regulation

Yes

T90 vs. T0

N.S.

N.S.

 

T90 vs. T30

N.S.

Up-regulation

 

SCAND1

T30 vs. T0

N.S.

N.S.

 

T90 vs. T0

De-methylation

Up-regulation

Yes

T90 vs. T30

De-methylation

N.S.

 

TTC7A

T30 vs. T0

N.S.

Down-regulation

 

T90 vs. T0

N.S.

N.S.

 

T90 vs. T30

De-methylation

Up-regulation

Yes

  1. Statistical significance of modulated methylation (de-methylated–methylated) or gene expression (up-regulated–down-regulated) is reported among the comparisons (T30 vs. T0, T90 vs. T0 and T90 vs. T30). N.S.: not statistically supported. The “Agreement” column was compiled only when both analysis outcomes were statistically supported.