Figure 3
Representative image of bioinformatics analyses. MicroRNome (GSE180674) and transcriptome (GSE180673) datasets from young rat VP were reanalyzed using the DESEq2 package. Differentially expressed targets were considered when p-value < 0.05 and Log2 Fold Change ≥|+ 0.66 ≤|− 0.66| (a). Up-set plot showing commonly deregulated miRNAs in the VP from the GLLP group and in patients with PCa (b). Heatmap showing the miRNAs expression profile shared between the VP from the GLLP group and PCa patients (c). Sequence alignment of commonly deregulated miRNAs in the VP from the GLLP group and in PCa samples. The sequences were obtained through the miRBase database (https://www.mirbase.org/) (d). Alluvial diagram showing the relationship between the main molecular pathways and enriched ontological terms for the predicted targets of miR-206, these analyzes were performed on the Enrichr platform (https://maayanlab.cloud/Enrichr/), considering p-value < 0.05 (e). Survival curves of PRAD patients (using TCGA data) show the impact of PLG, ZMAT3, and LFPL2 in the progression free PCa patients in altered (red) and unaltered (blue) risk (f). Immunohistochemical localization of PLG, ZMAT3, and LFPL2 in normal and PRAD tissues obtained from The Human Protein Atlas database (https://proteinatlas.org/) (g).
