Figure 4
Functional validation of miR-206 in PNT2 benign prostatic cells. Morphologic aspects of the PNT2 cells in the Mock (a), Scrambled (b), and miR206-Mimic (c) groups after 24 h of treatment. Expression profile of miR-206 in PNT2 cells in both experimental groups after 24 h of treatment (d). PLG (e), ESR1 (f), ESR2 (g), and AR (h) expression levels in PNT2 cells after 24 h of treatment in both experimental groups. Representative images of wound healing assay after 24, 48, and 72 h (i). Cellular wound closure after the transfection of PNT2 cells with Mock and miR206-Mimic groups after 0 h, 24 h, 48 h, and 72 h post-wound (j). Cell viability assay (MTT) after 24, 48, and 72 h (k). Mock group: cells treated with lipofectamine. Scrambled: cells treated with negative control mimic. miR206-Mimic group: cells treated with miR-206 mimic. Data are expressed as mean ± SD. *p < 0.05. Scale bar: A, B and C 50 µm; detail in A, B and C: 10 µm; j: 200 µm.
