Figure 1 | Scientific Reports

Figure 1

From: Investigating the role of aae-miR-34-5p in the regulation of juvenile hormone biosynthesis genes in the mosquito Aedes aegypti

Figure 1

The effect of miR-34 inhibitor on the expression of JH biosynthetic genes. (A) JH biosynthesis pathway modified from 30. The red circled enzymes are potential targets of miR-34. (B) The expression levels of miR-34 in the head + first thoracic segment of Ae. aegypti mosquitoes four days after injection with buffer (APS), negative control (NC) inhibitor, and miR-34 inhibitor assessed by RT-qPCR. The inhibition of miR-34 was confirmed. (C–F) The relative expression levels of four target genes in the JH biosynthetic pathway (red circled in A) in the head + first thoracic segment of Ae. aegypti mosquitoes treated as in (B) assessed by RT-qPCR. One-way ANOVA with Tukey’s post hoc analysis was used to compare the treatments. The error bars represent standard error of mean (SEM) of biological replicates each represented by a data point. ns, not significant; *, p < 0.05; **, p < 0.01; ****, p < 0.0001. (G) Fluorescent microscopy of CA incubated with a small RNA conjugated with Alexa Fluor 555.

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