Figure 2 | Scientific Reports

Figure 2

From: Investigating the role of aae-miR-34-5p in the regulation of juvenile hormone biosynthesis genes in the mosquito Aedes aegypti

Figure 2

Assessment of direct interactions of miR-34 with the predicted target sites in Thiolase, FALDH (2 sites), and FPPase. The target sites were cloned downstream of the GFP gene (Fig. S1), and co-transfected with miR-34 mimic or the negative control (NC) mimic into Sf9 cells. GFP transcript levels were assessed two days after transfection by RT-qPCR. t-test was used to compare each paired treatment. The error bars represent standard error of mean (SEM) of biological replicates each represented by a data point. ns, not significant; ***, p < 0.001; ****, p < 0.0001.

Back to article page