Figure 3

A comparison of the HV after the BI correction. (a) The brightness of each nucleus. (b) The brightness distribution of adenocarcinoma cells. In this nucleus (red circle), the number of pixels with a brightness of 106 was the highest, and this was defined as the brightness index (BI). (c) A comparison of the HV after BI correction across histological types. There was a significant difference between cancer and non-cancerous cells (Student's t test; **P < 0.01). Data are shown as the mean ± SD. Sq squamous cell carcinoma, Ad adenocarcinoma, Sm small cell carcinoma, Ci ciliated columnar epithelial cell.