Figure 2

MARCKS increases its localization to the endosome following LPS stimulation. (A, D) Immunofluorescent staining of MARCKS (red) in macrophages before and after LPS stimulation. (B, E) Immunofluorescence staining of EEA1, the early endosome marker (purple) in macrophages before and after LPS stimulation. (C, F) Images merged to show the co-localization of MARCKS and endosome. (A–F) The nuclei were stained with Hoechst (blue). (G) The percentages of colocalization of MARCKS and EEA1 were calculated. Data are represented as mean ± SEM and *p < 0.05 (one-way ANOVA).