Figure 5
From: Engineered CHO cells as a novel AAV production platform for gene therapy delivery
Analytical characterization of rAAVs produced in the CHO–HV–C1 clone. (a) Western blot of VP1, VP2 and VP3 capsid proteins from either purified rAAV6.2-GFP or rAAV9-GFP vectors produced with different MOIs in CHO–HV–C1 cells. (b) Mini transmission electron (Mini-TEM) micrograph of purified rAAV6.2-GFP. (c) Mini-TEM micrograph of purified rAAV9-GFP. White arrows indicate average full capsids, and black arrows indicate average non-full (empty) capsids. (d) The rAAV capsid ratio detection using CE-SDS method.
