Figure 6
From: Engineered CHO cells as a novel AAV production platform for gene therapy delivery
Infectivity of rAAVs produced in CHO–HV–C1 cells compared to HEK293. (a) Infectivity of purified rAAV6.2-GFP vector produced in either CHO–HV–C1 clone using rHSV-1 co-infection and/or HEK293 cells using triple transfection via TCID50. Data (n = 2 per sample) were analyzed with two-tailed Mann–Whitney test and expressed as mean ± SD. Individual biological replicates are shown for each group in the bar graph. (b) In vitro transduction of Ad293 cells with rAAV6.2-GFP produced in either CHO–HV–C1 cells using rHSV-1 co-infection or HEK293 cells using triple transfection. Mock infection of cells used PBS alone.
