Table 1 Descriptive statistics for the comparison between MS1 and MS2 measurements in DIA mode.
From: Q-RAI data-independent acquisition for lipidomic quantitative profiling
| Â | Positive Mode | Negative Mode | ||
|---|---|---|---|---|
Fixed Windows (20 (+ 1) Da) | Variable Windows | Fixed Windows (20 (+ 1) Da) | Variable Windows | |
MS1 | ||||
 Total Number of Features | 215 | 220 | 112 | 108 |
 Number of Features with RSD < 30% | 209 (97.21%) | 217 (98.64%) | 112 (100%) | 107 (99.07%) |
 Median RSD for Features with RSD < 30% | 2.43 | 2.37 | 3.04 | 2.06 |
 Number of Features with RSD > 30% | 6 (2.79%) | 3 (1.36%) | 0 (0%) | 1 (0.93%) |
MS2 | ||||
 Total Number of Fragments | 1982 | 2038 | 1078 | 1040 |
 Number of Fragments with RSD < 30% | 1150 (58.02%) | 1317 (64.62%) | 471 (43.69%) | 523 (50.29%) |
 Median RSD for Fragments with RSD < 30% | 8.44 | 7.16 | 10.96 | 9.45 |
 Number of MS1 Features with MS2 Fragments of RSD < 30% | 210 (95.45%) | 217 (98.64%) | 109 (97.32%) | 107 (99.07%) |
 Number of Fragments with RSD > 30% | 573 (28.91%) | 540 (26.50%) | 316 (29.31%) | 301 (28.94%) |
 Number of Non-Quantifiable Fragments | 259 (13.07%) | 181 (8.88%) | 291 (26.99%) | 216 (20.77%) |
