Table 2 Descriptive statistics for the comparison between measurements acquired on a Q-TOF by MS1 (DIA) and MS2 (DIA), or on a QQQ by MRM, for a six-point dilution series of a lipid extract from commercially available human plasma.
From: Q-RAI data-independent acquisition for lipidomic quantitative profiling
|  | Injection Volume (μL) | |||||
|---|---|---|---|---|---|---|
0.25 | 0.5 | 1 | 2 | 4 | 8 | |
DIA MS1 | ||||||
 Total Number of Features | 106 | |||||
  Median RSD (%) | 3.80 | 2.35 | 1.82 | 1.09 | 1.08 | 0.92 |
 Number of Features with RSD > 30% | 1 (0.94%) | 0 (0%) | 0 (0%) | 3 (2.83%) | 2 (1.89%) | 1 (0.94%) |
  Median R2 | 0.990 | |||||
 Number of Features with R2 < 0.8 | 5 (4.72%) | |||||
DIA MS2 | ||||||
 Total Number of Quantified Fragments | 651 out of 1007 (64.65% of all fragments) | |||||
  Median RSD (%) | 15.15 | 11.02 | 8.27 | 6.06 | 5.42 | 4.74 |
 Number of Fragments with RSD > 30% | 167 (25.65%) | 108 (16.59%) | 73 (11.21%) | 61 (9.37%) | 37 (5.68%) | 58 (8.91%) |
  Median R2 | 0.957 | |||||
 Number of Fragments with R2 < 0.8 | 143 (21.97%) | |||||
MRM | ||||||
 Total Number of Transitions | 287 | |||||
  Median RSD (%) | 5.74 | 1.71 | 1.41 | 1.68 | 1.38 | 5.51 |
 Number of Transitions with RSD > 30% | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) |
  Median R2 | 0.997 | |||||
 Number of Transitions with R2 < 0.8 | 0 (0%) | |||||
