Figure 6

Expression patterns of head-determination, hormone-related and germ-line genes involved in the stolonization process, quantified by real-time qRT PCR. Anterior and posterior body parts are indicated by blue and orange, respectively. (b) Expression patterns of head-determination genes six3, otx, and pax6. (c) Expression patterns of hormone-related genes, FAMeT [Farnesoic acid methyl transferase], Kr-h1 [Krüppel homolog 1], and EcR [Ecdysone receptor]. (d) Expression patterns of germ-line genes, vasa, piwi, and nanos. The vertical axes show relative expression levels against the selected reference gene (RP49), and horizontal axes show stolonization stages. Blue and orange bars, respectively, represent anterior and posterior body parts. Different letters on bars indicate significant differences (Tukey’s test, P < 0.05). Error bars indicate standard deviations.