Figure 6 | Scientific Reports

Figure 6

From: Low oral dose of 4-methylumbelliferone reduces glial scar but is insufficient to induce functional recovery after spinal cord injury

Figure 6

4-MU treatment reduced glial scar area surrounding the lesion site. (A) Representative fluorescent images showing lesion epicentre (0 mm), above (− 4, − 2 mm) and below (+ 2, + 4 mm) the lesion, stained for glial fibrillary acidic protein (GFAP) in placebo and 4-MU treated group with chronic spinal cord injury. Dotted lines show the border area of the lesion cavity in 4-MU treated group and GFAP positive area in placebo group. Scale bar: 200 µm. Diagram of uninjured spinal cord at top left showing the direction of the cross section in (A), created with BioRender.com; (B) magnified images (yellow square in A) showing structural change of the glial scar tissue after 4-MU treatment compared to placebo treated animals. Scale bar 30 µm; (C) bar graph showing area of the glial scar around the central cavity performed in the GFAP stained histochemical images using ImageJ software. Values are plotted as mean ± SEM; ****p < 0.0001 by two-way ANOVA, Sidak post-hoc test. (n = 4 animals per group). (D) Representative images of Luxol Fast Blue staining showing the lesion extension in a rostro-caudal direction. Scale bar 200 µm.

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