Figure 7

4-MU treatment leads to changes of cell and ECM composition around the lesion scar (Th8-9), above (Th5-6) and below (Th10-11) lesion. (A–E) Representative confocal images showing the 4-MU-mediated effect on scar-forming cells and components using different markers—(A) nestin and GFAP were used to visualise scar-forming astrocytes. (B) Iba-1 to visualise microglia/macrophages. (C) NG2 to visualise oligodendrocyte progenitor cells (OPCs). (D) CS-56 to examine the changes in CS sulfations. (E) Collagen 1a to visualise meninges and fibroblasts. All insets show magnified views of the staining. Scale bar 200 µm for the overview image and 50 µm for the insets. (F) Quantification of (A–E). Bar graphs show intensities per section throughout the spinal cord, except for Iba-1 staining where the number of Iba-1 positive cells per mm was counted. Individual data are shown with their mean ± SEM (n = 3 animals per group). p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, by two-way ANOVA, Sidak's multiple comparison test.