Figure 7
From: Fluorescent molecular rotors as versatile in situ sensors for protein quantitation
BCA vs. FMR protein concentration analysis of an unknown: (A) Bicinchoninic acid protein concentration assay identified unknown protein concentration to be 0.898Â mg/mL. (B) Out-lying point (A; black arrow) was removed and the BCA data replotted, determining unknown protein concentration to be 0.683Â mg/mL (C) FMR emission spectra data and (D) endpoint data determined unknown protein concentration to be 0.680Â mg/mL and 0.687Â mg/mL respectively.
