Figure 1 | Scientific Reports

Figure 1

From: A novel test for type-I allergy based on crosslink formation of immunoglobulin-E receptors by allergen-specific immunoglobulin-E antibodies and an allergen

Figure 1

A schematic showing the principle of AlphaCL to detect anti-DNP IgE Abs. When each bead comes into proximity by crosslinking of the allergen-specific IgE Abs bound to FcεRIα conjugated on each bead with an allergen, the acceptor beads can receive the singlet oxygen that is released from the donor beads by excitation at 680 nm. The energy generated by singlet oxygen in the acceptor beads results in a sharp emission peak at 615 nm. DNP, 2,4-dinitrophenylated.

Back to article page